Journal: Frontiers in Microbiology
Article Title: RmCP, a cerato-platanin protein from Rigidoporus microporus , induces defense responses during interaction with Hevea brasiliensis
doi: 10.3389/fmicb.2025.1553350
Figure Lengend Snippet: The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were stained with trypan blue at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
Article Snippet: Leaf tissues were immersed in 0.5% trypan blue stain solution (Nacalai Tesque, Japan) for an hour followed by rinsing in absolute ethanol.
Techniques: Activity Assay, Control, Staining, Light Microscopy